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METHYLENE BLUE SENSITIZED PHOTOINACTIVATION OF E. COLI RIBOSOMES: EFFECT ON THE RNA AND PROTEIN COMPONENTS †
Author(s) -
Singh Harwant,
Ewing Donald D.
Publication year - 1978
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1978.tb06967.x
Subject(s) - ribosome , ribosomal rna , rna , ribosomal protein , protein biosynthesis , methylene blue , microbiology and biotechnology , 23s ribosomal rna , 50s , chemistry , biology , biophysics , biochemistry , gene , photocatalysis , catalysis
— Methylene blue sensitizes photoinactivation of E. coli ribosomes. This photoinactivation shows dose‐dependent minor changes in the ribosome particles. The ribosomal RNA extracted from irradiated ribosomes also shows small changes. On gel electrophoresis the 23S ribosomal RNA band decreases in intensity at low doses with a corresponding increase in the minor band which moves slightly faster than the 23S band. The appearance of this minor band is dose‐dependent. The other major ribosomal component (16S ribosomal RNA) is relatively stable. The S6 protein is one of the first proteins to decrease in intensity at low doses followed by fairly marked changes in some of the other proteins, at high doses.