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EFFECT OF SODIUM AZIDE ON PHOTODYNAMIC INDUCTION OF GENETIC CHANGES IN YEAST
Author(s) -
Kobayashi Katsumi
Publication year - 1978
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1978.tb06965.x
Subject(s) - acridine orange , sodium azide , mitosis , saccharomyces cerevisiae , locus (genetics) , yeast , acridine , chemistry , gene , sensitization , azide , biology , dna , genetics , microbiology and biotechnology , biochemistry , apoptosis , organic chemistry , immunology
— In view of the increasing attention to 1 O 2 ( 1 Δ g ) participation in the photodynamic action, different types of genetic changes in Saccharomyces cerevisiae by acridine orange sensitization were compared with respect to the response to N 3 ‐ , a well known quencher of 1 O 2 . The induction of mitotic crossing over with respect to ade 2 locus and mitotic gene conversion at trp 5 locus were suppressed by the addition of N 3 ‐ suggesting the involvement of 1 O 2 as a major intermediate. However, the induction of reverse mutation at ilv 1 was only slightly suppressed. These results may indicate that there are two types of photodynamic DNA damage; one is produced via 1 O 2 and the other via non‐ 1 O 2 reaction pathway which lead to mitotic gene conversion and mitotic crossing over, and to mutation, respectively.