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A BLUE LIGHT REACTION INVOLVING FLAVTN NUCLEOTIDES AND PLASTOCYANIN FROM PROTOSIPHON BOTRYOIDES
Author(s) -
O'Kelley Joseph C.,
Hardman John K.
Publication year - 1977
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1977.tb09128.x
Subject(s) - flavoprotein , flavin group , chemistry , plastocyanin , pigment , nucleotide , photochemistry , tryptophan , riboflavin , amino acid , biochemistry , organic chemistry , enzyme , photosystem i , chloroplast , gene
— A pigment that absorbs yellow light (580 nm) appears in a cell‐free preparation of Protosiphon when it is irradiated with blue light (430 nm) in the presence of flavoprotein from Photosiphon or flavin nucleotides. The pigment has been isolated and purified. It is a protein with a molecular weight of approximately 9000 daltons. An amino acid analysis reveals that it lacks arginine and tryptophan. It is colorless when reduced and blue (absorbs at 580 nm) when oxidized. Its normal oxidation–reduction potential is + 0.37 V. It contains one copper ion per molecule and has other properties that match those of plastocyanin isolated from green cells of other species.