Premium
APPLICATION OF PHOTODYNAMIC OXIDATION TO THE DISINFECTION OF TAPWATER, SEA WATER, AND SEWAGE CONTAMINATED WITH POLIOVIRUS
Author(s) -
Gerba Charles P.,
Wallis Craig,
Melnick Joseph L.
Publication year - 1977
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1977.tb07521.x
Subject(s) - chemistry , poliovirus , methylene blue , seawater , virus , sewage , contamination , chromatography , microbiology and biotechnology , nuclear chemistry , virology , biochemistry , photocatalysis , catalysis , biology , environmental engineering , ecology , engineering
. Poliovirus when added to tapwater, sewage or seawater was readily photoinactivated by methylene blue and visible light. Typically, almost 2.5 logs of virus could be inactivated upon a 5‐min exposure to 670 nm light (20 W/m 2 ) in solutions containing 13 μ M methylene blue at pH 10.0. A biphasic inactivation curve was produced for poliovirus, regardless of dye concentration, pH, temperature, sensitization time, nature of suspending solution or sequence of light exposure. These results indicated that a multi‐hit inactivation event was occurring. Preincubation of the dye‐virus mixture at 24°C increased the rate of virus photoinactivation. Dye concentrations above 26 μ M have little advantage in increasing the amount of virus photoinactivated. Significant inactivation of the virus in the dark occurred at high dye concentrations (52–130μ M ).