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PHOTODYNAMIC INACTIVATION OF YEAST SENSITIZED BY EOSIN Y
Author(s) -
Cohn G. E.,
Tseng H. Y.
Publication year - 1977
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1977.tb07516.x
Subject(s) - eosin y , eosin , yeast , chemistry , irradiation , saccharomyces cerevisiae , biophysics , photochemistry , photodynamic therapy , extracellular , h&e stain , biochemistry , staining , biology , photocatalysis , genetics , organic chemistry , nuclear physics , catalysis , physics
. Wild‐type diploid yeast has been irradiated with visible light in the presence of eosin Y to investigate the photodynamic inactivation of this model eukaryote. Light, eosin Y and oxygen were all required for substantial inactivation, and no dark recovery was detected. Long periods of irradiation were required for greater than 90% inactivation, corresponding to a very small low‐dose quantum yield. Neither binding nor uptake of the dye by yeast was detected. Corrections for the photooxidative bleaching of eosin Y during irradiation indicate that bleaching causes a significant reduction in the apparent rate of inactivation. The results suggest that eosin Y acts as an extracellular sensitizer where the likelihood of damage to the cell envelope is enhanced.