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CYTOCHROME b IN PLASMA MEMBRANE ENRICHED FRACTIONS FROM SEVERAL PHOTORESPONSIVE ORGANISMS*
Author(s) -
Schmidt W.,
Thomson K.,
Butler W. L.
Publication year - 1977
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1977.tb07506.x
Subject(s) - neurospora crassa , neurospora , phycomyces , membrane , cytochrome , absorbance , flavin group , phycomyces blakesleeanus , chemistry , dictyostelium discoideum , biochemistry , biophysics , pigment , photochemistry , biology , chromatography , organic chemistry , enzyme , gene , mutant
. Plasma membrane enriched fractions from Phycomyces blakesleeanus, Neurospora crassa and Dictyostelium discoideum were examined spectrally. All showed a membrane bound b ‐type cytochrome and the presence of some flavin but the membrane preparations did not exhibit any light‐induced absorbance changes. It was concluded that most of the flavin which might have been associated with the membranes in vivo had been washed off during the preparation procedures. However, the reduced‐minus‐oxidized difference spectrum of the cytochrome b in the plasma membrane enriched fraction from Neurospora was very similar to light‐minus‐dark difference spectrum for the photoreduced cytochrome b in whole cells of Neurospora , both spectra being measured at –196°C to enhance spectral discrimination.