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FLUORESCENCE ANALYSIS ON SOLID SURFACES *
Author(s) -
Guilbault George G.
Publication year - 1977
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1977.tb07361.x
Subject(s) - fluorometer , chromatography , fluorescence , chemistry , silicone rubber , thin layer chromatography , potassium bromide , electrophoresis , analytical chemistry (journal) , organic chemistry , optics , physics
— One of the recent uses of fluorescence has been in the assay of substances directly on solid surfaces such as paper chromatograms, thin‐layer chromatography (TLC) plates, potassium bromide disks, electrophoresis strips, and silicone‐rubber pads. Chromatography and electrophoresis are widely used analytical techniques and it is not surprising that fluorometric methods are used in conjunction with them. Advantages are increased sensitivity and rapidity of analysis. In this paper the use of fluorescence in the assay of substances after separation on paper and TLC chromatograms, as well as the direct assay of substances on a solid surface placed directly in a commercial fluorometer, are discussed. Particular emphasis is placed on the direct analysis of enzymatic reactions.