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PHOTOSENSITIZING EFFECTS OF 8‐METHOXYPSORALEN ON THE SKIN OF HAIRLESS MICE—I. FORMATION OF INTERSTRAND CROSS‐LINKS IN EPIDERMAL DNA
Author(s) -
Ley R. D.,
Grube D. D.,
Fry R. J. M.
Publication year - 1977
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1977.tb06909.x
Subject(s) - hairless , dna , chemistry , biophysics , microbiology and biotechnology , biology , biochemistry
— The photomediated induction of interstrand cross‐links by 8‐methoxypsoralen has been measured in the epidermal DNA of hairless mice. Equivalent efficiencies for cross‐link induction were determined for HRS/J/Anl and SKH: hairless‐1 mice. A wavelength dependence on the relative efficiency of cross‐link induction was observed; a broad spectrum light source, 300–400 nm, was approximately 5 times more effective in cross‐link formation than a 365 nm light source. Repeated exposure to 8‐methoxypsoralen followed by ultraviolet light, 5 times a week for 6 weeks, altered epidermal thickness and resulted in a decreased efficiency for DNA cross‐link formation.