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PHOTOCHEMICAL CROSSLINKING OF ATP TO HISTONE H4
Author(s) -
Sperling Joseph
Publication year - 1976
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1976.tb07255.x
Subject(s) - moiety , irradiation , photochemistry , chemistry , acetone , monomer , covalent bond , yield (engineering) , quantum yield , stereochemistry , organic chemistry , materials science , polymer , physics , quantum mechanics , nuclear physics , metallurgy , fluorescence
— A covalent crosslink occurs between histone H4 and the adenine moiety of ATP, when the complex they form is irradiated with UV light of Λ > 290 nm in the presence of acetone. Within 1 h of irradiation a 48% yield of crosslinked product is thus obtained. It is also shown that in the photosensitized reaction, 80% of the crosslinked product is monomeric, whereas protein precipitation and aggregation occur as a result of direct irradiation with 254 nm light.