Premium
PHOTOSENSITIZATION OF HUMAN DIPLOID CELL CULTURES BY INTRACELLULAR FLAVINS AND PROTECTION BY ANTIOXIDANTS
Author(s) -
Pereira Olivia M.,
Smith James R.,
Packer Lester
Publication year - 1976
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1976.tb06817.x
Subject(s) - ascorbic acid , flavin group , lipid peroxidation , wi 38 , population , intracellular , biochemistry , vitamin , vitamin c , cell , chemistry , cell culture , ultraviolet light , antioxidant , biology , microbiology and biotechnology , ploidy , genetics , photochemistry , enzyme , food science , gene , demography , sociology
— The damaging effects of near ultraviolet and visible light on WI‐38 human diploid lung fibroblasts were investigated. WI‐38 cells in culture were killed by light doses ranging from 2 to 10 × 10 3 W/m 2 h. There was an inverse correlation between culture age, i.e. population doubling level and photosensitivity. However, this effect could not be related to capacity for DNA synthesis and cell division. Flavins were clearly implicated as endogenous photosensitizers, and antioxidants such as d, l ‐α‐tocopherol (vitamin E), BHT and ascorbic acid were found to afford the cells protection from light damage. Furthermore, products of lipid peroxidation could be detected in cell homogenates irradiated in the presence of ribofiavin.