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THE ELECTROPHOTOLUMINESCENCE OF CHLOROPLASTS
Author(s) -
Ellenson James L.,
Sauer Kenneth
Publication year - 1976
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1976.tb06782.x
Subject(s) - electron transport chain , electric field , electron , saturation (graph theory) , atomic physics , ion , luminescence , physics , photosystem ii , chemistry , photosystem i , field (mathematics) , pulse (music) , perturbation (astronomy) , analytical chemistry (journal) , photosynthesis , optics , voltage , quantum mechanics , biochemistry , mathematics , chromatography , combinatorics , pure mathematics
— Delayed light emission emanating from preilluminated chloroplasts can be perturbed with pulsed DC electric fields (200–4000 V cm ‐1 ), The perturbation produces a strong stimulation of chlorophyll luminescence. During the field perturbation the stimulated emission rises to a maximum, typically within 100μs. and then decays. Two kinetic components, R (rapid) and S (slow)†, are distinguished on the basis of their rise and decay times and their field‐dependence. The R component increases exponentially at high fields, decays within 100–300μs during the field pulse and collapses with t 1/2 = 15 μs at the end of the field pulse. The S component occurs at low fields, exhibits near saturation at 500 V cm ‐1 , decays with t 1/2 about 3 ms during the field pulse, and collapses with t 1/2 = 38μs at the end of the field pulse. Studies using inhibitors, ionophores, electron donors and electron acceptors associate the R component with ion transport processes. The relation to electron transport associated with Photosystem II is discussed.