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INFLUENCE OF THE ENVIRONMENT ON THE EXCITATION WAVELENGTH DEPENDENCE OF THE FLUORESCENCE QUANTUM YIELD OF INDOLE
Author(s) -
Tatischeff I.,
Klein R.
Publication year - 1975
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1975.tb06740.x
Subject(s) - quantum yield , chemistry , fluorescence , indole test , excited state , acetonitrile , photochemistry , absorption (acoustics) , cyclohexane , tryptophan , solvent , wavelength , analytical chemistry (journal) , aqueous solution , yield (engineering) , butanol , ethanol , materials science , stereochemistry , atomic physics , optics , organic chemistry , biochemistry , physics , optoelectronics , amino acid , composite material , metallurgy
— The influence of excitation wavelength, pH, oxygen and solvents, upon fluorescence quantum yields, were measured for insole Indole in neutral aqueous solution exhibits the same wavelength dependence as tryptophan, which indicates that COO‐ absorption is not responsible for the effect. Parameters such as pH and oxygen influence only the absolute fluorescence quantum yields but not their relative variation with wavelength, indicating competition with fluorescence emission for S1 deactivation. without any influence upon deactivation of higher excited states. In contrast, solvents exhibit a specific influence upon the wavelength dependence; for indole, the decrease of fluorescence yield excited around 215 nm, compared with the yield in the first absorption band is about 40% in water, 10% in acetonitrile, 70% in n‐hexane and cyclohexane, whereas no appreciable decrease occurs in methanol, ethanol or n‐butanol. These observations, together with the Stokes shifts of the emission spectra, may be well correlated with Kosower's Z‐values, expressing microscopic solvent‐solute interactions.