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AN ANALYSIS OF PORPHYRIDIUM ABSORPTION BANDS WITH A DIGITAL SPECTROPHOTOMETER
Author(s) -
Leclerc J. C.,
Hoarau J.,
GuérinDumartrait E.
Publication year - 1975
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1975.tb06719.x
Subject(s) - absorbance , absorption (acoustics) , analytical chemistry (journal) , resolution (logic) , gaussian , absorption spectroscopy , position (finance) , spectral line , high resolution , derivative (finance) , optics , chemistry , biological system , physics , chromatography , remote sensing , computer science , biology , geology , artificial intelligence , computational chemistry , finance , astronomy , economics , financial economics
— –Numerical absorbance values treated by a computer allow one to easily obtain precise absorption spectra from intact cells or extracts. An attempt has been made to determine the number and position of the individual bands by the difference spectra and the fourth derivative method. These two methods resulted in an accurate curve resolution with single Gaussian components and yielded relative proportions of chl a forms in Porphyridium.