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ISOLATION AND PARTIAL CHARACTERIZATION OF THE PHOTOCHEMICAL REACTION CENTER OF CHROMATIUM VINOSUM (STRAIN D)
Author(s) -
Lin Lily,
Thornber J. Philip
Publication year - 1975
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1975.tb06718.x
Subject(s) - chromatium , photosynthetic reaction centre , chemistry , photochemistry , strain (injury) , bacteriochlorophyll , hydroxylapatite , photosynthesis , organic chemistry , biology , pigment , biochemistry , enzyme , electron transfer , anatomy
— –A preparation of the photochemical reaction center of Chromatiwn has been obtained by chromatography of lauryldimethylamineoxide‐solubilized chromatophores on hydroxylapatite and Sepharose columns. The procedure has yielded a reaction center preparation from both carotenoid‐containing and carotenoid‐deficient Chromatium cells. Preliminary analysis of the isolated component indicates that the photochemical reaction center of the Thiorhodaceae is homologous to that of the Athiorhodaceae. In particular, the near infrared absorption spectrum of the Chromatium reaction center preparation shows the same triple‐peaked spectrum observed for reaction center preparations from the Athiorhodaceae. The Chromatium preparation undergoes a rapid light‐induced oxidation and dark reduction of the reaction center. The ratio of the reaction center to the two membrane‐bound cytochromes (cytochrome c 552 and c 555 ) is greatly increased over the ratio observed in chromatophores or in other previously isolated, reaction center‐enriched subchromatophore fractions of this organism.