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ANALYSIS OF THE BINDING OF PHYTOCHROME TO PARTICULATE FRACTIONS
Author(s) -
Schäfer Eberhard
Publication year - 1975
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1975.tb06651.x
Subject(s) - phytochrome , in vivo , cooperativity , biophysics , coleoptile , in vitro , cooperative binding , function (biology) , chemistry , binding site , biochemistry , biology , botany , microbiology and biotechnology , red light , genetics
— The binding of phytochrome to receptor sites in a particulate fraction of maize coleoptiles has been studied as a function of the level of far‐red‐absorbing phytochrome (P fr ) offered in vivo and in vitro. Evidence is presented that the binding is cooperative. The degree of cooperativity expressed by the Hill coefficient of the binding function is the same (1–6) both in vivo and in vitro , whereas the Hill coefficient of the state function in vivo is significantly higher (2‐1). The highest Hill coefficient (3–5) was found for the in vitro binding function in squash hooks.