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STUDY OF THE EFFECTS OF ULTRAVIOLET LIGHT ON BIOMEMBRANES—IV. THE EFFECT OF OXYGEN ON UV‐INDUCED HEMOLYSIS AND LIPID PHOTOPEROXIDATION IN RAT ERYTHROCYTES AND LIPOSOMES
Author(s) -
Roshchupkin D. I.,
Pelenitsyn A. B.,
Potapenko A. Ya.,
Talitsky V. V.,
Vladimirov Yu. A.
Publication year - 1975
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1975.tb06629.x
Subject(s) - hemolysis , chemistry , liposome , membrane , lipid peroxidation , irradiation , biophysics , lecithin , erythrocyte membrane , membrane lipids , photochemistry , oxygen , photodissociation , protoporphyrin , biochemistry , antioxidant , biology , organic chemistry , immunology , physics , porphyrin , nuclear physics
— Hemolysis induced by irradiation with ultraviolet (UV) light at 254 nm showed a pronounced oxygen effect: under irradiation in vacuum, the rate of hemolysis was decreased by an order of magnitude. Irradiation at 254 nm in air but not under vacuum caused the peroxidation of erythrocyte membrane lipids. These results suggest that membrane lipid photoperoxidation is one of the causative factors of UV hemolysis. Irradiation at different wavelengths showed that UV‐induced lipid photoperoxidation in erythrocyte membranes developed while the antioxidant α‐tocopherol was directly photooxidized. It is shown that the process of lipid photolysis in erythrocyte membranes involves sensitization, possibly by protoporphyrin, whose presence in liposomes accelerates the photoperoxidation at 254 and 365 nm of unsaturated fatty acid residues in lecithin. Possible mechanisms of photochemical damage to erythrocyte membranes are discussed.