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FLUORESCENCE AND PHOTOCHEMISTRY OF OLIGOCYTIDYLIC AND POLYCYTIDYLIC ACIDS IN AQUEOUS SOLUTION
Author(s) -
Vigny Paul,
Favre Alain
Publication year - 1974
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1974.tb06586.x
Subject(s) - chemistry , fluorescence , adduct , protonation , photochemistry , aqueous solution , monomer , absorbance , excimer , polymer , organic chemistry , ion , quantum mechanics , physics , chromatography
—In addition to the monomer‐like fluorescence, a long‐wavelength emission (Λ max em = 410 nm) has been detected in the dinucleoside 5′‐5′ pyrophosphate (CppC) at room temperature. This emission looks very similar to that previously reported for the acidic forms of Poly C (Poly C. Poly C + and Poly I. Poly C. Poly C + ). Only the monomer‐like emission (Λ max em = 330 nm) can be detected in neutral Poly C, acidic CppC, and the neutral or protonated forms of the dinucleoside phosphate CpC.A correlation between the room temperature fluorescence of oligo and polycytidylic acids and their photochemical behaviour is found. Irradiation of all the polymeric samples at both neutral and acid pH results in the formation of minor photoproducts. They have been characterized by their absorbance (in the range 300–400 nm) and their fluorescence spectra. The same product is obtained in all cases where the monomer‐like fluorescence only is detected. Distinct products are formed in neutral CppC and in the acidic Poly C forms. The results are discussed with respect to the conformation of the oligo and polycytidylic acids and possible relationships between the 410–420 nm emission and adduct formation. An excimer is proposed as a common, intermediate excited state in both radiative deactivation and adduct formation in neutral CppC and the acidic Poly C forms.