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THE FATE OF PYRIMIDINE DIMERS IN THE DNA OF ULTRAVIOLET‐IRRADIATED CHINESE HAMSTER CELLS
Author(s) -
Meyn Raymond E.,
Vizard Douglas L.,
Hewitt Roger R.,
Humphrey Ronald M.
Publication year - 1974
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1974.tb06570.x
Subject(s) - pyrimidine dimer , chinese hamster ovary cell , dimer , ultraviolet light , dna , ultraviolet , chinese hamster , polynucleotide , hamster , dna replication , biology , dna repair , chemistry , microbiology and biotechnology , biophysics , photochemistry , biochemistry , cell culture , genetics , materials science , optoelectronics , organic chemistry
—As an aid to understanding the relationship between dimer repair and cellular recovery, we have studied dimer removal and replication of dimer‐containing DNA in Chinese hamster ovary (CHO) cells irradiated with ultraviolet light (254 nm). These investigations demonstrated that (1) dimers are not excised as polynucleotides of less than 500,000 mol. wt, (2) fractionation of the ultraviolet dose does not enhance dimer excision, (3) dimer‐containing DNA is replicated in ultraviolet‐irradiated CHO cells, and (4) the dimers are conserved in the replicated DNA. These findings support the proposed mechanism of bypass of photoproducts during DNA replication in mammalian cells.