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ULTRAVIOLET INACTIVATION OF THE ABILITY OF E. COLI TO SUPPORT THE GROWTH OF PHAGE T7: AN ACTION SPECTRUM
Author(s) -
Day Rufus S.,
Muel Bernard
Publication year - 1974
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1974.tb06555.x
Subject(s) - action spectrum , bacteriophage , escherichia coli , ultraviolet , wavelength , nucleic acid , chemistry , cytidine , biophysics , biology , photochemistry , biochemistry , physics , optoelectronics , enzyme , gene
—The action spectrum for inactivation of the capacity of stationary‐phase Escherichia coli B to support the growth of T7 bacteriophage was measured, using radiation 240–405 nm in wavelength. The action spectrum has a minimum at 254 nm, a maximum at 267 nm, and a shoulder between 313 and 334 nm, followed by a decrease at longer wavelengths, indicating that nucleic acid is probably not the target for capacity inactivation. Deoxycytidine, cytidine, thiamine, and some proteins have absorption spectra similar to the far‐ultraviolet (240–300 nm) portion of this action spectrum.