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IN VITRO PHOTOREACTIVATION OF ULTRAVIOLET‐INACTIVATED RIBONUCLEIC ACID FROM TOBACCO MOSAIC VIRUS
Author(s) -
Hurter Jakob,
Gordon Milton P.,
Kirwan James P.,
McLaren A. Douglas
Publication year - 1974
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1974.tb06497.x
Subject(s) - tobacco mosaic virus , photolyase , nicotiana tabacum , infectivity , biology , rna , ultraviolet light , in vitro , virus , biochemistry , virology , chemistry , microbiology and biotechnology , dna , dna repair , photochemistry , gene
— Treatment of ultraviolet‐inactivated tobacco mosaic virus ribonucleic acid (TMV–RNA) with extracts obtained from the local lesion host, Nicotiana tabacum var. Xanthi , n.c., and simultaneous illumination at 365 nm results in up to a four‐fold increase in infectivity over non‐illuminated controls. The active material in the extract appears to be associated with protein, based on its inactivation by boiling, precipitation with ammonium sulfate, and exclusion from Bio‐Rad P100 polyacrylamide. Partially purified DNA photoreactivating enzyme from yeast or pinto bean has no activity on ultraviolet‐irradiated TMV–RNA.