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ACTION SPECTRUM FOR ENZYMATIC PHOTOREACTIVATION IN MAIZE *
Author(s) -
Ikenaoa M.,
Kondo S.,
Fujii T.
Publication year - 1974
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1974.tb06485.x
Subject(s) - photolyase , action spectrum , enzyme , ultraviolet , in vitro , pollen , dna , biology , ammonium sulfate , chemistry , biochemistry , botany , dna repair , physics , chromatography , quantum mechanics
— An ammonium‐sulfate‐precipitable (35–70 per cent) fraction in extracts of Zea mays pollen contains photoreactivating enzyme that reactivates the transforming ability of ultraviolet‐inactivated Hemophilus influenzae DNA. The action spectrum of in vitro photoreactivation with the enzyme has a broad single maximum at 385 nm. Parallel to the in vitro response, in vivo photo‐reactivation of ultraviolet‐induced premutational damage in pollen of this plant is more efficient at 405 nm than at 334 nm. From these results we conclude that photoreactivation of ultraviolet‐induced mutational damage in maize pollen is chiefly of the direct enzymatic type.