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CELLULAR REACTIVATION OF ULTRAVIOLET‐IRRADIATED HUMAN ADENOVIRUS 2 IN NORMAL AND XERODERMA PIGMENTOSUM FIBROBLASTS
Author(s) -
DayIII Rufus S.
Publication year - 1974
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1974.tb06467.x
Subject(s) - xeroderma pigmentosum , fibroblast , ultraviolet light , virus , cell culture , biology , virology , population , microbiology and biotechnology , cell , chemistry , genetics , dna repair , dna , medicine , photochemistry , environmental health
Abstract— A plaque assay for adenovirus 2 on normal human fibroblasts has been developed and used to measure the survival of ultraviolet‐irradiated virus on six human fibroblast cell lines. When four xeroderma pigmentosum cell lines were used as viral hosts, an average of one lethal event per virus in the viral population was made with 10, 15, 62, and 78 J m ‐2 respectively, while using two normal cell lines as hosts, 197 and 205 J m ‐2 were required to inflict the same damage. These differences are attributed to the known repair deficiency of xeroderma pigmentosum cells, and are discussed in the light of previous data obtained using other animal viruses.