Premium
PHOTO‐OXIDATION OF LYSOZYME IN THE PRESENCE OF METHYLENE BLUE: A PROBABLE MECHANISM
Author(s) -
Churakova N. I.,
Kravchenko N. A.,
Serebryakov E. P.,
Lavrov I. A.,
Kaversneva E. D.
Publication year - 1973
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1973.tb06412.x
Subject(s) - lysozyme , singlet oxygen , methylene blue , chemistry , methylene , photochemistry , oxygen , chitin , singlet state , organic chemistry , catalysis , photocatalysis , excited state , biochemistry , chitosan , physics , nuclear physics
— In order to elucidate the mechanism of the photo‐oxidation of lysozyme sensitized by methylene blue this enzyme was oxidized by singlet oxygen generated by a high–frequency discharge in gaseous oxygen. The action of singlet oxygen on lysozyme gives products identical with those obtained upon dye‐sensitized oxidation with respect to their affinity to chitin, their amino acid content, their UV spectra and their enzymic activity. It is concluded that photo‐oxidation of lysozyme in the presence of methylene blue proceeds according to the singlet‐oxygen mechanism.