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PHYTOCHROME INTERMEDIATES IN VIVO –II. CHARACTERISATION OF INTERMEDIATES BY DIFFERENCE SPECTROPHOTOMETRY
Author(s) -
Spruit C. J. P.,
Kendrick R. E.
Publication year - 1973
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1973.tb06405.x
Subject(s) - epicotyl , irradiation , chemistry , in vivo , pisum , photochemistry , biochemistry , biology , botany , physics , microbiology and biotechnology , nuclear physics , seedling
— In epicotyl tissue of Pisum , irradiation of P r at – 196°C forms a stable product P 698 , whereas P fr forms a stable product P 650 . On warming P 698 , dark transformation to P r predominates. On warming P 650 to – 70°C an intermediate P 690 is formed which bleaches on further warming to –10°C. When tissue is cooled to –196°C during actinic irradiation, difference spectra for subsequent warming to –10°C indicate that P r , P fr and an intermediate P 710 are formed from weakly absorbing intermediates. Complete photoconversion of P r to P fr is not possible at temperatures below –5°C. As the temperature is reduced, the amount of P fr produced from P r decreases, while P 710 increases. P 710 can be photoconverted at –20°C and above, ultimately forming P r , but in contrast to P fr it is not photoconvertible at –196°C.