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PHOTOCHEMICAL LABELING OF YEAST ALCOHOL DEHYDROGENASE WITH AN AZIDE ANALOG OF NAD +
Author(s) -
Hixson Stephen S.,
Hixson Susan H.
Publication year - 1973
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1973.tb06403.x
Subject(s) - nad+ kinase , azide , chemistry , reagent , alcohol dehydrogenase , photochemistry , photodissociation , yeast , covalent bond , alcohol , enzyme , biochemistry , organic chemistry
— 3‐Azidopyridine adenine dinucleotide, an azide analog of NAD + , has been synthesized as a potentially general photochemical labeling reagent for the active sites of NAD‐dependent dehydrogenases. The analog is a competitive inhibitor of NAD reduction by yeast alcohol dehydrogenase (YADH). Upon photolysis of the 3 H‐analog in the presence of YADH, 7% of the label becomes covalently bound. The results are discussed in terms of desired properties of a photochemical labeling reagent.