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EFFECTS OF PHOTOSYSTEM II INHIBITORS ON ELECTRON PARAMAGNETIC RESONANCE SIGNAL II OF SPINACH CHLOROPLASTS
Author(s) -
LOZIER RICHARD H.,
BUTLER WARREN L.
Publication year - 1973
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1973.tb06342.x
Subject(s) - dcmu , photosystem ii , spinach , hydroxylamine , chemistry , photosystem i , chloroplast , photochemistry , electron paramagnetic resonance , tricine , photosynthesis , nuclear magnetic resonance , biochemistry , physics , gene
— In isolated spinach chloroplasts the light‐induced electron paramagnetic resonance signal (signal II) associated with the oxygen evolving photosystem (photosystem II) decays slowly and incompletely in the dark. Tris‐washing, hydroxylamine, or carbonylcyanide m ‐chlorophenylhydrazone (CCCP) enhance the decay of signal II, which can still be induced by red (645 nm) but not by far‐red (735 nm) radiation. Although 3‐(3,4‐dichlorophenyl)‐1,1‐dimethylurea (DCMU) alone has no effect on signal II, it blocks the induction of signal II in the presence of hydroxylamine or CCCP. These data suggest that signal II is an indicator of an oxidized intermediate on the water‐splitting side of photosystem II.