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PHOTOLYSIS OF POLYRIBOBROMOURIDYLIC ACID
Author(s) -
EHRLICH MELANIE,
RILEY MONICA
Publication year - 1972
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1972.tb06307.x
Subject(s) - chemistry , absorbance , photodissociation , hydrolysis , irradiation , polymer , photochemistry , hydrate , alkaline hydrolysis , chromatography , organic chemistry , physics , nuclear physics
— Photolysis of polyribobromouridylic acid with 313 nm light at neutral pH caused extensive debromination and a loss of A 280 (280–nm absorbance) without comparable increase in A 260 . At an exposure of 190μE/cm 2 , strand breakage occurred on the average of one break every 170 BrU residues. Little if any pyrimidine hydrate was produced. Exhaustive RNase hydrolysis of photolysed polymer gave a mixture of mononucleotides and oligonucleotides. The mononucleotide fraction was found to be composed of unaltered BrUMP and contained little if any UMP. Irradiation of the polymer at alkaline pH caused little or no debromination or spectral change.