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PHOTOACTIVATION OF UROCANASE IN PSEUDOMONAS PUTIDA: POSSIBLE CONFORMATIONAL CHANGE DURING ACTIVATION
Author(s) -
HUG DANIEL H.,
ROTH DENNIS
Publication year - 1972
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1972.tb06292.x
Subject(s) - pseudomonas putida , chromophore , conformational change , action spectrum , chemistry , enzyme , biophysics , photochemistry , stereochemistry , biochemistry , biology
Earlier studies of urocanase indicated that it is converted to an inactive form in resting cells of Pseudomonas putida . When cell extracts are irradiated by ultraviolet (u.v.) light, the enzyme is activated[1]. The chromophore is closely associated with the enzyme [ 1 ,2]. The action spectrum for photoactivation showed that, although near‐u.v. light was effective, the most efficient wavelengths were at 275–280 nm[3]. The study we present here suggests that urocanase undergoes a conformational change upon photoactivation.