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CHANGES IN TOTAL DIFFUSE SPECTRAL REFLECTANCE OF AMINO ACIDS AND PROTEINS AFTER ULTRAVIOLET IRRADIATION
Author(s) -
ANGLIN J. H.,
SAYRE R. M.,
BATTEN W. H.
Publication year - 1972
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1972.tb06266.x
Subject(s) - amino acid , irradiation , histidine , cystine , reflectivity , chemistry , diffuse reflection , ultraviolet , diffuse reflectance infrared fourier transform , photochemistry , biochemistry , materials science , cysteine , optics , enzyme , photocatalysis , physics , optoelectronics , nuclear physics , catalysis
— Total diffuse reflectance spectra of air‐dried surfaces of free and neutralized amino acid preparations before and after irradiation in vitro are reported. It was found that some free or neutralized amino acid surfaces underwent modification which resulted in changes in the diffuse reflectance spectra after U.V. exposure. It is suggested that these reflectance changes result from transformations in the side chains of the amino acids and that the transformations may differ from those occurring when amino acids in solution are irradiated. Histidine, cystine, hydroxyproline and some protein surfaces showed changes in reflectance of 330–400 nm light similar to those reported in skin after U.V. irradiation in viuo.