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FLASH PHOTOLYSIS STUDIES OF PROTEINS AND INDOLE DERIVATIVES IN SOLUTION
Author(s) -
SUBRAMANYAN V.,
TOLLIN G.
Publication year - 1972
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1972.tb06256.x
Subject(s) - flash photolysis , indole test , chemistry , photochemistry , aqueous solution , flash (photography) , triplet state , residue (chemistry) , oxygen , combinatorial chemistry , stereochemistry , kinetics , organic chemistry , molecule , reaction rate constant , optics , physics , quantum mechanics
— Transients obtained upon flash photolysis of a number of proteins in aqueous solution appear to derive from electron ejection from tryptophyl residue side chains. These decay by a second order process. Oxygen is an effective quencher for the protein transients but is less so for the flash‐induced signals obtained from simple indole derivatives. Experiments using other quenchers indicate that the signals are not due to an indole triplet state, but that the triplet state may be a precursor of the flash‐induced metastabie species. Compounds which bind to the active site of chymotrypsin were found to exert only non‐specific effects on the flash‐induced signals.