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THE INFLUENCE OF NUTRITION ON THE DNA CONTENT OF ESCHERICHZA COLI AND ITS RESPONSE TO ULTRAVIOLET LIGHT
Author(s) -
WEBB S. J.,
ROKOSH D. A.
Publication year - 1971
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1971.tb06188.x
Subject(s) - escherichia coli , lactose , dna , amino acid , biochemistry , locus (genetics) , nitrogen , ultraviolet , bacteria , dna synthesis , carbon fibers , ultraviolet light , chemistry , dna replication , biology , gene , genetics , photochemistry , organic chemistry , physics , materials science , quantum mechanics , composite number , composite material
— The influence of nutrition on the sensitivity of Escherichia coli 15 T‐ to ultraviolet light (u.v.) and the synthesis of DNA has been studied. Growth in media containing glucose or NH, + has been found to endow cells with a greater resistance to lethal u.v. damage than those grown in media containing succinate or amino acids, respectively. In addition, the sensitivity of the lactose ( lac ) locus of the DNA to mutagenic damage has been found to be altered by changes in the carbon supply but not by changes in the nitrogen source, while the sensitivity of loci controlling amino acid synthesis was altered by changes in the nitrogen source but not in the carbon source. Cells fed with glucose or NH 4 + have been found to possess more DNA than cells fed with succinate or amino acids, respectively. The data indicate that the type of carbon and nitrogen supplied to the cells will determine whether or not set regions of the DNA will undergo more than one round of replication. The presence in the cell of identical genetic loci either in duplicate or in multiples, directed by the particular types of carbon and nitrogen supplied, is suggested to be, in part, the reason why an alteration in nutrition is able to influence the sensitivity of bacterial cells to radiation.