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DELAYED‐LIGHT STUDIES ON PHOTOSYNTHETIC ENERGY CONVERSION‐III. EFFECT OF 3‐(3,4‐DICHLOROPHENYL)‐1,1 ‐DIMETHYL UREA ON THE MILLISECOND EMISSION FROM CHLOROPLASTS PERFORMING PHOTOREDUCTION OF FERRICYANIDE *
Author(s) -
BERTSCH W.,
WEST JANET,
HILL R.
Publication year - 1971
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1971.tb06170.x
Subject(s) - photochemistry , dcmu , chemistry , ferricyanide , electron transport chain , chlorophyll , metastability , singlet state , millisecond , fluorescence , chlorophyll a , photosynthesis , excited state , atomic physics , photosystem ii , inorganic chemistry , physics , organic chemistry , optics , biochemistry , astronomy
— How does a plant convert electronic excitation of chlorophyll into stable chemical potential? The time scales of fluorescence (10 ‐ ‐ 9 sec) and steady‐state enzymatic turnover (10– 2 sec) indicate that energy storage must be involved. Millisecond delayed singlet emission from chlorophyll allows measurement of metastable energy storage at Photoreaction II. Activation of noncyclic electron transport results in more rapid decay and in increase of emission at 10 ‐ ‐ 3 sec, both effects being inhibited by the poison DCMU. These results can be explained by at least three different models of the reaction center: the oxidized chlorophyll model, the chlorophyll triplet model, and the two‐quantum electron‐hole model.