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PHOTOBIOLOGICAL EFFECTS OF METAL IONS. PHOTOSENSITIZING ACTION OF URANYL ACETATE ON Escherichia coli K‐12 *
Author(s) -
NEELY W. C.,
ELLIS S. P.,
CODY R. M.
Publication year - 1971
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1971.tb06145.x
Subject(s) - uranyl , uranyl acetate , chemistry , escherichia coli , ion , metal , metal ions in aqueous solution , nuclear chemistry , chelation , penetration (warfare) , inorganic chemistry , biochemistry , organic chemistry , biology , staining , gene , genetics , operations research , engineering
— The Uranyl acetate sensitized killing of Escherichia coli K‐12 by a light source approximating sunlight in both intensity and wavelength distribution is demonstrated to occur at a concentration of 5.0 × 10O ‐4 M uranyl acetate (pH 7.0). The photosensitized killing was evident after 150 min and almost complete within 320 min. Auxotrophic mutants were isolated from cultures incubated in both light and dark for 160 min at this concentration of uranyl acetate. Binding of UO 2 2+ to E. coli is shown to occur with 82% of the UO 2 2+ ions in a 5 × 10 ‐4 M solution (pH 7.0) being bound to the cell wall. In the dark as well as at other pH values the extent of binding was much less. Most of the binding occurred in a time less than 30 min. The observation of rapid binding but delayed photosensitization is attributed to the necessity for penetration of uranyl ions into the cells' interior to effect photosensitization.