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PHOTOSENSITIZED DEGRADATION OF C‐PHYCOCYANIN *
Author(s) -
BELLIN JUDITH S.,
GERGEL CAROL A.
Publication year - 1971
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1971.tb06131.x
Subject(s) - phycocyanin , chromophore , homogeneous , ultracentrifuge , chemistry , action spectrum , photochemistry , fluorescence , degradation (telecommunications) , chromatography , visible spectrum , cyanobacteria , biology , materials science , optics , telecommunications , genetics , physics , bacteria , computer science , thermodynamics , optoelectronics
— –Irradiation with visible light in the presence of allylthiourea causes the transformation of phycocyanin into a ‘purple’ chromoprotein absorbing maximally at 565 and 605 nm. This reaction is irreversible, and not affected by the presence of oxygen. The product is homogeneous on electrophoresis, ultracentrifugation and chromatography, and has a molecular weight one third that of the starting material. Although apparently physically homogeneous, the action spectrum for fluorescence suggests that the ‘purple’ compound may consist of two two chromophores associated with protein moieties that are indistinguishable.