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DNA REPAIR IN ULTRAVIOLET LIGHT IRRADIATED HeLa CELLS AND ITS REVERSIBLE INHIBITION BY HYDROXYUREA
Author(s) -
BENHUR E.,
BENISHAI R.
Publication year - 1971
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1971.tb06121.x
Subject(s) - pyrimidine dimer , incubation , dna , hela , microbiology and biotechnology , nucleotide excision repair , acriflavine , ultraviolet light , chemistry , dna synthesis , dimer , irradiation , sucrose , dna repair , biochemistry , biology , cell , photochemistry , physics , organic chemistry , nuclear physics
— –The repair of u.v. damaged DNA in HeLa cells can be detected using the alkaline sucrose gradient technique. As a result of pyrimidine dimer excision single strand breaks are produced in DNA of irradiated cells. Rejoining of these breaks occurs during an 8 hr post‐irradiation incubation period and is prevented by hydroxyurea and acriflavine. The inhibition of repair by hydroxyurea can be reversed by a mixture of all 4 deoxyribonucleosides at a concentration that does not reverse the inhibition of total DNA synthesis.