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CHANGES IN FLUORESCENCE SPECTRA OF CHLOROPLASTS INDUCED BY A NATURALLY‐OCCURRING FACTOR
Author(s) -
NATHANSON B.,
BRODY M.
Publication year - 1970
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1970.tb06079.x
Subject(s) - fluorescence , chloroplast , chemistry , emission spectrum , photochemistry , absorption (acoustics) , absorption spectroscopy , chlorophyll a , chlorophyll , incubation , analytical chemistry (journal) , spectral line , materials science , chromatography , biochemistry , optics , organic chemistry , physics , astronomy , composite material , gene
— –Fluorescence emission changes (measured at – 196°C) of Ricinus chloroplasts incubated in isolation medium, and of chloroplasts from algae and higher plants incubated in Ricinus leaf extract, are described. Such incubation results in a transformation of the three‐banded emission spectrum (F735, F698, F685) into a virtually one‐banded spectrum, with maximum at 698 nm. That these changes are a consequence of the conversion (deaggregation) of the form of chlorophyll giving rise to F735 into a form contributing to fluorescence at 698 nm is suggested on the basis of room temperature absorption and low temperature fluorescence excitation studies, made concomitantly with the low temperature emission studies.