Premium
AN ANALYSIS OF FOURTH DERIVATIVE SPECTRA
Author(s) -
BUTLER W. L.,
HOPKINS D. W.
Publication year - 1970
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1970.tb06077.x
Subject(s) - derivative (finance) , signal (programming language) , noise (video) , spectral line , signal to noise ratio (imaging) , absorption spectroscopy , absorption (acoustics) , order (exchange) , mathematics , analytical chemistry (journal) , biological system , physics , algorithm , chemistry , computer science , statistics , optics , chromatography , biology , artificial intelligence , quantum mechanics , financial economics , economics , image (mathematics) , programming language , finance
— –Fourth derivative spectra of chloroplast at – 196°C, obtained by four sequential differentiations of the absorption spectrum, were examined to determine the conditions for optimal signal‐to‐noise ratio and resolution. An appreciable improvement in the signal‐to‐noise ratio was found when the four differentiating intervals were nearly but not exactly equal. The process of differentiating digitized data with the computer was explored in order to define the rules for optimization of the fourth derivative curves.