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THE EFFECT OF ULTRAVIOLET LIGHT ON RNA METABOLISM IN BROMOURACIL DEOXYRIBOSIDE‐GROWN HeLa CELLS *
Author(s) -
PAINTER R. B.,
GOODMAN M. G.,
REISNER B. L.
Publication year - 1970
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1970.tb06037.x
Subject(s) - rna , hela , cytoplasm , uracil , uridine , metabolism , biochemistry , biology , dna , nucleus , microbiology and biotechnology , irradiation , cell , chemistry , physics , gene , nuclear physics
— The presence of 5‐bromouracil deoxyriboside (BrUdR) in the DNA of HeLa cells has profound effects on RNA metabolism after u.v. irradiation. In normally grown cells 200 ergs/mm 2 depresses RNA synthesis by about 30 per cent while in BrUdR‐grown cells the same exposure to u.v. depresses RNA synthesis by 95 per cent. When BrUdR‐grown cells are u.v. ‐irradiated after being labeled with 3 H‐uridine, the normal autoradiographic pattern, where label shifts from nucleus to cytoplasm, fails to occur. Also, in lieu of the increase in RNA specific activity that is observed in unirradiated cells for a few hours after 20‐min pulse‐labeling, there occurs a constant decrease in specific activity after the irradiation.