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SOME OBSERVATIONS ON THE LETHAL EFFECTS OF NEAR‐ULTRAVIOLET LIGHT ON ESCHERICHIA COLI , COMPARED WITH THE LETHAL EFFECTS OF FAR‐ULTRAVIOLET LIGHT
Author(s) -
PEAK M. J.
Publication year - 1970
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1970.tb06031.x
Subject(s) - escherichia coli , irradiation , acriflavine , near ultraviolet , nutrient agar , ultraviolet , ultraviolet light , agar , ultraviolet irradiation , far ultraviolet , biology , microbiology and biotechnology , chemistry , bacteria , photochemistry , biochemistry , genetics , optics , physics , astronomy , gene , nuclear physics , spectral line
— Near‐ultraviolet light (365.5 nm) reduces the ability of Escherichia coli B/r and B 8‐1 , to form colonies on nutrient agar after irradiation. This lethal effect is distinct from that obtained after far‐u.v. irradiation (253.7 nm) because the far‐u.v. sensitive and resistant strains are equally susceptible to near‐u.v. Variation in susceptibility to ultraviolet light during growth is more marked for near‐u.v. than for far‐u.v. The number of survivors after near‐u.v. irradiation of log phase cells is affected by several post‐irradiation treatments; more cells survive if growth immediately after irradiation occurs at higher temperatures (unlike far‐u.v.). Also, the presence of acriflavine and caffeine in the nutrient agar decreases the number of survivors (in common with far‐u.v.).