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PHOTOREACTIVATION OF ESCHERICHIA COLI B s‐3 AFTER IN ACTIVATION BY 313 nm RADIATION IN THE PRESENCE OF ACETONE
Author(s) -
MENNIGMANN HORSTDIETER,
WACKER ADOLF
Publication year - 1970
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1970.tb06001.x
Subject(s) - photolyase , acetone , thymine , escherichia coli , photochemistry , pyrimidine dimer , population , chemistry , radiation , irradiation , biophysics , biology , physics , biochemistry , dna , optics , dna repair , gene , demography , nuclear physics , sociology
— The colony‐forming ability of E. coli B s‐3 can be inactivated by light of 313 nm wavelength in an acetone‐sensitized photochemical reaction. This ability can subsequently be restored quantitatively by illumination with photoreactivating light. A small fraction of the population cannot be inactivated; this is assumed to be due to a complete dark repair of the lesions, whatever the dose of radiation has been. Thus, such triplet energy‐transfer experiments can successfully be applied to whole cells. Since thymine dimers are formed almost exclusively, this suggests a new way of studying these lesions in relation to the biologically observable effects.