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SEROLOGICAL PROPERTIES OF PORCINE TRYPSIN SUBSEQUENT TO INACTIVATION BY VARIOUS METHODS *
Author(s) -
Ghiron C. A.,
Barrett J. T.,
Humber J. Y.
Publication year - 1968
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1968.tb05832.x
Subject(s) - autolysis (biology) , trypsin , antiserum , chemistry , precipitin , biochemistry , chromatography , enzyme , biology , antibody , immunology
— Porcine trypsin was inactivated by various methods (heat, autolysis, photodynamic action, ultraviolet light, and gamma‐radiation) and the inactivated products were tested for their ability to precipitate porcine trypsin antiserum using the capillary fluid precipitin method. It was found that the chemical and structural modifications incurred with irradiation, sufficient to inactivate porcine trypsin, in contrast to heat and autolysis, did not interfere with those parts of the protein surface required for immunochemical reactivity.