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PHOTOREACTIVATING ENZYME FROM NEUROSPORA CRASSA *
Author(s) -
Terry Claude E.,
Setlow Jane K.
Publication year - 1967
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1967.tb08893.x
Subject(s) - neurospora , photolyase , neurospora crassa , escherichia coli , biology , action spectrum , yeast , biochemistry , dna , enzyme , pyrimidine dimer , microbiology and biotechnology , genetics , dna repair , biophysics , gene , mutant
— Extracts of Neurospora crassa contain photoreactivating enzyme by the criteria of ability to split thymine‐containing dimers and to increase the transforming ability of u.v.‐irradiated Hemophilus influenzae DNA. The latter activity is heat‐labile and is destroyed by trypsin. The action spectrum of such in vitro photoreactivation is a simple one (with a single maximum at 405 nm in the range 313 to 436 nm), differing from the more complicated in vitro spectra for yeast and Escherichia coli. However, the in vitro Neurospora spectrum coincides closely with the in vivo spectrum for this organism, suggesting that there is little or no “indirect” photoreactivation in Neurospora. It is concluded that the Neurospora photoreactivating enzyme is probably of a different type than those of yeast and Escherichia coli.