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PHOTOREVERSAL OF ACRIFLAVINE INHIBITION OF DEOXYRIBONUCLEIC ACID REPLICATION IN ULTRAVIOLET LIGHT‐EXPOSED BACTERIA
Author(s) -
Doudney C. O.
Publication year - 1967
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1967.tb08770.x
Subject(s) - acriflavine , dna , pyrimidine dimer , dna synthesis , escherichia coli , ultraviolet light , dna replication , strain (injury) , biology , bacteria , biophysics , chemistry , biochemistry , microbiology and biotechnology , dna repair , genetics , photochemistry , gene , anatomy
With a strain of Escherichia coli lacking the capacity for photoenzymatic splitting of the pyrimidine dimer, acriflavine prevents restoration of DNA replication after u. v. exposure even after photoreversal treatment. With a revertant strain possessing capacity for photoreversal response, light exposure prior to addition of the dye eliminates a large part of the blockage of DNA synthesis by the dye. These results show that the presence of dimercontaining regions in the DNA prevents restoration of DNA replication when the dye is present. However, the non‐photoreversibility of some of the u.v.‐damage responsible for blockage of DNA synthesis by the dye suggests that acriflavine also interacts with lesions not restituted by the photoreactivating enzyme.