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MICROSURGICAL APPLICATION OF A PULSED RUBY LASER IN EXPERIMENTAL CELL BIOLOGY
Author(s) -
Leppard Gary G.,
Wacker Gerhart
Publication year - 1966
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1966.tb05809.x
Subject(s) - microscope , laser , instrumentation (computer programming) , optics , pulsed laser , biological tissue , biological specimen , ruby laser , laser beams , microscopy , biomedical engineering , materials science , computer science , physics , medicine , operating system
— The microsurgical application of a pulsed ruby laser used in conjunction with a multi‐objective microscope is discussed. Some design criteria and various technical and practical problems relating to such use of a laser‐microscope complex are considered. Control of the volumetric energy density at the specimen by means of the instrumentation variables is established on the basis of systematic studies conducted on a stained protein “model tissue”. The instrumentation variables offering the greatest degree of control are the beam energy, the objective focal length, and the degree of defocusing of the laser beam. Results of the “model tissue” study enable one to predict in advance the size and severity of destructive effect that will be produced in thin layers of green plant tissue using a particular combination of variables. A judicious extension of these results enables single cell and intracellular microsurgery to be performed with precision. In addition, various biological tests and experiments were conducted in order to determine the applicability of a laser‐microscope system to biological research and to delineate its limitations.