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STUDIES ON THE INACTIVATION OF TOBACCO MOSAIC VIRUS BY ULTRAVIOLET LIGHT*,‡,§
Author(s) -
Goddard Judy,
Streeter David,
Weber Celia,
Gordon Milton P.
Publication year - 1966
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1966.tb05783.x
Subject(s) - tobacco mosaic virus , nucleic acid , protein subunit , chemistry , guanidine , ultraviolet light , hydrochloride , rna , biochemistry , virus , ultraviolet irradiation , acetic acid , sodium dodecyl sulfate , sodium , amino acid , covalent bond , microbiology and biotechnology , biology , irradiation , virology , photochemistry , gene , organic chemistry , physics , nuclear physics
— The irradiation of TMV with u.v. light of 2537 Å wavelength results in the binding of protein subunits to the RNA. These bound subunits are stable towards warm sodium dodecyl‐sulfate; however, the binding is not covalent since the subunits are removed by 66% acetic acid, guanidine hydrochloride, or phenol. Approximately one protein subunit is bound per lethal biological ‘hit’. The virus and the nucleic acid extracted from irradiated virus show virtually identical rates of inactivation.