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ACTION SPECTRA FOR PHOTOREACTIVATION OF MUTATION TO PROTOTROPHY IN STRAINS OF ESCHERICHIA COLI POSSESSING AND LACKING PHOTOREACTIVATING‐ENZYME ACTIVITY *
Author(s) -
Kondo S.,
Jagger J.
Publication year - 1966
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1966.tb05780.x
Subject(s) - photolyase , escherichia coli , auxotrophy , action spectrum , biology , mutation , chemistry , genetics , biophysics , dna repair , dna , gene
— The action spectrum and dose‐rate dependence for photoreactivation of mutation to prototrophy in late‐lag‐phase cultures of Escherichia coli H 3 r30 (which lacks active photo‐reactivating enzyme) are roughly similar to those for photoprotection from killing in other strains. It is suggested that photoreactivation of this mutation in H/r30 is an indirect effect, similar in mechanism to photoprotection. The action spectrum and dose‐rate dependence for photoreactivation of mutation to prototrophy in late‐lag‐phase cultures of E. coli H 3 r30‐R (which possesses active photoreactivating enzyme) are roughly similar to those for photoreactivation of killing in most other strains. It is suggested that photoreactivation of this mutation in H/r30‐R is a direct effect at long wavelengths, but that there is an indirect component at short wavelengths. A quite different interpretation of these data is noted. Finally, it is found that, under the conditions of these experiments, indirect photoreactivation of killing in H/r30 and H/r30‐R is weak or nonexistent.