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MITOTIC DELAY AND THE APPARENT SYNERGISM OF FAR‐RED RADIATION AND X‐RAYS IN THE PRODUCTION OF CHROMOSOMAL ABBERATIONS *
Author(s) -
Wolff Sheldon,
Luippold H. E.
Publication year - 1965
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1965.tb09757.x
Subject(s) - mitosis , metaphase , far red , population , irradiation , chromosome , red cell , biology , genetics , radiation , biophysics , physics , optics , botany , red light , gene , medicine , nuclear physics , environmental health
— The apparent synergism of far‐red radiation and X‐rays in the induction of chromosome aberrations has been found to be an artifact caused by a far‐red‐induced mitotic delay. Far red neither increases the number of X‐ray‐induced breaks nor decreases the amount of restitution (repair). It merely shifts the time of arrival of cells at metaphase where they can be scored. At any single given time after X‐irradiation, a different population of cells will be scored if far‐red as well as X‐rays are given. Since various portions of the cell cycle differ in their sensitivity to X‐rays, the far‐red‐induced mitotic delay can lead to apparent increases in aberration yields if cells are scored at only one time after X‐irradiation. When the whole cell cycle is sampled, however, far‐red is found not to affect the yield of aberrations.