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A METHOD OF ASSAYING IN VIVO THE SURVIVAL OF ULTRA VIOLET‐IRRADIATED TUMOUR CELLS
Author(s) -
Stevens G. H.,
Roe E. M. F.
Publication year - 1965
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1965.tb07917.x
Subject(s) - irradiation , survival analysis , ascites , cell survival , inoculation , in vivo , biology , ultraviolet irradiation , andrology , cell culture , immunology , medicine , physics , genetics , nuclear physics
— The growth of ultraviolet‐irradiated ascites tumour cells in adult animals which have received a total‐body dose of x‐radiation appears to be a suitable method to obtain data on the survival of the treated tumour cells, if the dose of total‐body radiation and its time before tumour cell inoculation are chosen correctly. In assessing tumour cell survival, the analysis of the time of appearance of the resulting solid tumours, using the linear relationship between the reciprocal of this time and the logarithm of the number of cells injected is preferred to probit or to Reed‐Muench analysis of the number of tumour‐takes. The 1/T method of analysis yields more precise estimates of the survival ratios and greater economy in the use of host animals without a disproportionate increase in the labour involved in obtaining the data. An application of this method to the determination of survival‐ratios of u.v.‐irradiated Landschütz ascites tumour cells is described.