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A SOLUBLE PROTEIN‐CHLOROPHYLL COMPLEX FROM SPINACH CHLOROPLASTS 11. THE PHYSICAL AND PHOTOCHEMICAL PROPERTIES OF THE COMPLEX *
Author(s) -
Kahn Joseph S.,
Bannister Thomas T.
Publication year - 1965
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1965.tb05722.x
Subject(s) - quantum yield , chemistry , photochemistry , molar absorptivity , autoxidation , chlorophyll , chloroplast , chlorophyll a , spinach , ferricyanide , yield (engineering) , fluorescence , inorganic chemistry , biochemistry , organic chemistry , materials science , quantum mechanics , optics , metallurgy , gene , physics
— Some physical and photochemical properties of a proteinxhlorophyll complex which can be extracted from spinach chloroplasts with dilute Triton X‐100, have been measured. The molar extinction coefficient at the red maximum (671 mμ ) is 6.8 × 10 4 . Solutions of the complex are weakly fluorescent, having a quantum yield of about 0.01. The complex undergoes photoautooxidation in the presence of oxygen with a quantum yield of about 0.2 × 10 ‐ ‐ 4 , and sensitizes the autooxidation of p ‐toluene‐diamine (quantum yield about 1×10 ‐ ‐ 3 ) and of ascorbic acid (quantum yield 0.25). The complex‐catalyzed reduction of ferricyanide, in the presence of a protein‐carotene complex, was confirmed; its quantum yield is about 8 × 10 ‐ ‐ 4 . The protein‐chlorophyll complex does not undergo a Krasnovsky reaction, nor could sensitized reductions of dye by abscorbate be detected. When excess Triton is added to the protein‐chlorophyll complex, it is converted into a detergent colloid apparently identical to a previously described Tween colloid. The properties of the protein‐chlorophyll complex are compared with those of other states of chlorophyll in vivo and in vitro .