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THE QUANTUM YIELD OF THE CHEMILUMINESCENCE OF DIMETHYLBIACRIDYLIUM NITRATE AND THE MECHANISM OF ITS ENZYMICALLY INDUCED CHEMILUMINESCENCE*
Author(s) -
Totter John R.
Publication year - 1964
Publication title -
photochemistry and photobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.818
H-Index - 131
eISSN - 1751-1097
pISSN - 0031-8655
DOI - 10.1111/j.1751-1097.1964.tb08779.x
Subject(s) - acridone , chemiluminescence , chemistry , quantum yield , yield (engineering) , photochemistry , fluorescence , ion , hypoxanthine , solvent , molecule , organic chemistry , physics , materials science , quantum mechanics , metallurgy , enzyme
— Quantum yields for dimethylbiacridylium ion chemiluminescence based on the amount of methyl acridone formed by treatment with either H 2 O 2 or with xanthine oxidasehypoxanthine in 0.01 M Na 2 CO 3 at pH 10.4 and at 25°C were found to lie between 0.011 and 0.020 with an average of about 0.016.In mixed solvents containing pyridine and water or alcohol and water the emission spectrum of chemiluminescence of dimethylbiacridylium ions as well as those of diniethylbiacridene and its oxide were found to be identical with or very similar to the fluorescence of methyl acridone in the same solvent.A mechanism involving two successive two equivalent reductions of the diniethylbiacridylium ion to dimethylbiacridan followed by a radical attack and auto‐oxidation leading to a compound which can undergo a reverse aldol type reaction to yield one (or two) molecule of methylacridone and one of a somewhat more reduced form is suggested.The kinetic equation relating maximum intensity of chemiluminescence to dimethylbiacridylium ion, hypoxanthine, xanthine oxidase, H + and O 2 concentrations was derived from the scheme suggested and found to fit satisfactorily the data obtained from a series of experiments in which the quantum yield was also obtained.